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The mitochondrial DNA and Y chromosome suffer only minor mutations through centuries, thus can be used to establish the paternal line in males because only males have the Y chromosome and the maternal line in both males and females. In general, the test results showed that European ancestry is far more important than those students had thought it would be. Another significant portion came from the Portuguese Atlantic Island of Madeira. Conversely, in the Northeast, Center-West and Southeast, African ancestry was the second most prevalent. Brazil has the largest Lebanese and Syrian population outside the Levant region, Christians in the great majority. A consistent flux of German immigrants started to arrive to Southern Brazil, briefly interrupted by the Ragamuffin War , but the amount of Portuguese immigrants was much bigger during this period.

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